Bd Facsdiva Software Download 15
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Information recorded in the analysis phase is then used by the cytometer software for population discrimination, gate set up, compensation and fluorescence detection. The analysis of data is done using FACS Diva software bundled with the instrument. It consists of several accessible modules, each one with a dedicated set of tools, allowing direct manipulation by the user, such as: manual gating, quadrant or dotplot generation.
The analysis of a sample starts from a single cell suspension, usually prepared by incubating the cells with the desired fluorescent dyes for 30 minutes. Then the suspension is loaded into the flow cytometer and is measured. After performing preliminary checks and setting up the instrument, the mathematical and statistical algorithm is initiated. The resulting histogram is then displayed. These operations are very fast and can be done interactively by the user. It normally takes 3 to 5 minutes to prepare a sample and less than 20 minutes to obtain and display the first basic set of information. d2c66b5586
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